Chlamydia trachomatis (CT) is the most common, sexually transmitted bacterial disease in the United States infecting 2.8 million people annually. In the developmental cycle of CT, Scc4 is a unique enzyme that has dual function as a class I T3SS chaperone and a transcription factor. The Scc4 modulates transcription in CT by interacting with σ66 region 4 (initiation factor) and the β subunit of RNAP. This σ66RNAP:Scc4 complex can inhibit σ66-dependent transcription, which regulates the transition from reticulate bodies (RB) to elementary bodies (EB) in the developmental cycle of CT. Understanding Scc4’s activity as a transcription factor and the mechanism of σ66RNAP:Scc4 complex formation will contribute to therapeutic approaches for treating CT. We are investigating the binding kinetics and structural determinants of the σ66RNAP:Scc4 complex using nuclear magnetic resonance (NMR), surface plasmon resonance (SPR), and size exclusion chromatography (SEC). Changes in the oligomeric state of Scc4, Scc4 interactions with σ66 region 4 and β subunit of RNAP will be presented along with the progress toward the NMR, 3D structure determination of Scc4.

Mechanisms of σ66RNAP: Scc4 mediated transcriptional regulation in Chlamydia trachomatis

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.