Aflatoxins are toxic metabolites generated by Aspergillus flavus and Aspergillus parasiticus. These toxins have been reported to be associated with acute liver damage, liver cirrhosis, induction of tumors and teratogenic effects. The presence of aflatoxins in many agricultural products such as corn, nuts, grain, and spices has been a global concern by both regulatory departments and the public. It is important to develop a rapid sample preparation method for aflatoxins analysis in foods and body fluids in response to food emergency. Traditional immunoassay and solid-phase extraction (SPE) techniques have been used for extraction of aflatoxins in foods, however, those methods usually involve tedious conditioning steps and large amounts of organic solvents. Dispersive pipette extraction (DPX) is a type of newly developed SPE method that is easy, quick, and reliable. DPX eliminates the use of large volumes of organic solvents and is environmentally friendly. The current research employed sample preparation using DPX followed by high performance liquid chromatography (HPLC) analysis of Aflatoxins B1, B2, G1, G2, and M1. Mean recoveries for all targeted aflatoxins were determined to be above 80% with relative standard deviation of 13.3%, which showed good accuracy and precision. Matrix match calibration study was done to evaluate the linearity, limit of detection (LOD), limit of quantification (LOQ) of the current method. Good linearity was obtained for all aflatoxins with R2 value greater than 0.993. LOD and LOQ were found to be below 6.3 ppb and 19.0 ppb, respectively.

Development of rapid dispersive pipette extraction method for analysis of aflatoxins and major metabolite using high performance liquid chromatography (HPLC)

255th National Meeting (2018)

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