IgM and IgG antibodies are one of the fastest growing diagnostic and therapeutic candidates. Unlike IgGs, IgM antibodies cannot be purified by affinity chromatography due to their physiochemical properties. We show the purification of acidic IgMs with a new strong anion exchanger (AEX) for the capture and mixed-mode chromatography media for the subsequent polishing. The anion exchange material is optimized to purify larger proteins and shows high binding capacities for IgM molecules (~1000 kDa). The capture step removes process related impurities while the following polishing depletes these impurities further. The described purification strategy is simple and scalable. Initials studies resulted in a product purity of &gt;95% IgM (SDS PAGE). This purity makes the IgM suitable for diagnostic applications.<br/>

Acidic IgM purification using anion exchange and mixed mode chromatography

255th National Meeting (2018)

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