Heparin is the most widely used anticoagulant for a variety of medical procedures, such as cardiovascular and orthopedic surgeries. Despite heparin’s importance in the medical field the current methods of quantifying heparin, which include optical and electrochemical methods are costly, time consuming and limit point-of-care testing. Therefore, this study examines whether a paper based analytical device (PAD) with a cellphone app as a detector can be used to quantify heparin to overcome such limitations. The PAD is made using traditional optical membrane components, including an ion-exchanger and chromionophore, applied to a membrane filter. The responses using different chromoionophores (ETH 2412 and ETH 5294), membrane compositions (1-5 wt% tridodecylmethylammonium chloride and 1-5 wt% ETH 2412), pH (4-12), and time (2-30 min) were evaluated to optimize the method. The application of the optimized membrane to unfractionated heparin (UFH) and low molecular weight heparin (LMWH) will be discussed.

Characterization of a paper-based analytical device for low molecular weight and unfractionated heparin

255th National Meeting (2018)

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