Abstract Objective: Polyphenols in fruits exist in different forms such as exractable and non-extractable polyphenols (NEP). Extractable polyphenols (EP) can be further divided into free polyphenosl (FP), Base hydrolysable polyphenols (BHP), and acid hydrolysable polyphenols (AHP). There is no detailed information on the potential health effects of these different polyphenols, and the aim of this study was to establish the anti-inflammation and anti-cancer effects of different polyphenol fractions from blueberry. Methods and results: Freeze dried blueberry powder was subjected to different extraction methods and yielded four polyphenol fractions, namely FP, BHP, AHP, and NEP. Their anti-inflammatory effects were determined on lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophages. No noticeable toxic effect was observed from the four fractions, while significant inhibition on LPS-induced production of nitric oxide was resulted by the polyphenol fractions. Among the four extracts, AHP showed the strongest inhibtion, followed by NEP and FP, while BHP was much weaker than other three fractions. The anti-inflammation effects were associated with lowered expression level of pro-inflammatory proteins such as inducible nitric oxide synthase (iNOS) and cylcooxygenase-2(COX-2). Furthermore, AHP and NEP showed potent inhibition on the growth of HCT116 human colon cancer cells, which was due to cell cycle arrest and cellular apoptosis induced by AHP and NEP. In summary, polyphenols from blueberry had anti-inflammation and anticancer effect, and these effects were specific to different tractions of polyphenols. AHP from the conjugated polyphenols in blueberry showed most potent biological effects. Our results provided fundamental information for the potential application of blueberry polyphenols to promote human health.

Anti-inflammation and anti-cancer effect of polyphenols from blueberry (Vaccinium corymbosum L.)

255th National Meeting (2018)

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