Peroxidase is an enzyme, which can oxidize many different phenolic alcohols with hydrogen peroxide. The stability of unbuffered crude extract from white turnip (Brassica rapa subsp. rapa) peroxidase activity was assayed using a syringaldazine assay following 25 minutes in a water bath at temperatures between 20 oC and 80 oC. This activity was compared to assays with added 0.7 mM calcium chloride, 0.7 mM ethylene glycol, or both calcium chloride and ethylene glycol. The enzyme had the most reliable activity below 60 oC with and without additives. When heated with calcium chloride, the peroxidase maintained its greatest activity. The extract could not maintain reliable peroxidase activity in the presence of ethylene glycol above 40 oC, whether or not calcium chloride was added.

White turnip peroxidase enzymatic stability with calcium chloride and ethylene glycol

255th National Meeting (2018)

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