Recently, bionanocellulose (BNC) has gained a lot of attention for biomedical applications such as artificial skin development or blood vessels replacement. Komagataeibacter xylinus is one of the most effective bionanocellulose (BNC) producer which due to its properties, such as biocompatibility and ease of modification of the structure is regarded as appropriate material in scaffold production for tissue regeneration. The major impediment tissue engineers, is the limited porosity of the biopolymer, that makes the ingrowth of the eukaryotic cells impossible. One possible way to solve this problem is genetic modification of Komagataeibacter strains. Unfortunately, the current knowledge about molecular mechanisms of bionanocellulose biosynthesis and its regulation is still insufficient to change the properties and structure of obtained BNC membranes. We have examined genes encoding two transmembrane proteins that form a proton/sodium channel engaged in proton motive force (pmf) generation. Proteins A and B are orthologues of MotA/MotB – flagellum stator proteins and (TonB-) ExbB/ExbD, TolR/TolQ(-TolA), AglQ/AglR(-AglS) proteins functioning as transport energizers. The phenotype analysis of mutants A:kan and AB::kan has shown structural changes in BNC membranes. Here, will be presented the results of RNA sequencing of interruption mutants Komagataeibacter xylinus E25. The early insights suggest that tested genes have similar function like their orthologs.

RNA-seq analysis of Komagataeibacter xylinus E25 A:kan and AB::kan mutants

255th National Meeting (2018)

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