The behavior of 5-HT1B serotonin receptor was observed in N2a (murine neuroblastoma) cells to monitor intracellular trafficking. We examined the change in membrane of expression of 5-HT1B in N2a cells during differentiation. Previous studies have implicated an effect of selective serotonin reuptake inhibitors (SSRIs) on the membrane expression of 5-HT1B; therefore, understanding the trafficking of 5-HT1B could aid the generation of more effective SSRIs. N2a cells were transfected with pDNA expressing HA-5-HT1B and cells were serum-starved to promote differentiation, causing the transfected cells to develop axons and dendrites. Immunocytochemistry (ICC) was performed to locate 5-HT1B receptors in relation to these processes. Preliminary data shows that the longer the cells were starved, the more differentiated the cells became, up to 12 days. The membrane expression of 5-HT1B was also observed in differentiated cells, and examined over the course of differentiation. We hope that studying the membrane expression of 5-HT1B will help elucidate the effect of SSRIs on the molecular mechanisms of psychological disorders.

Membrane expression of 5-HT1B receptors in N2a cells in response to SSRIs

255th National Meeting (2018)

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