Caries is one of the most common childhood diseases and presents a serious health problem. The formation often starts in the early childhood and continues in the permanent dentition.<br/>The aim of this study is therefore, to gain a better understanding of physiological processes on the tooth surface and to identify differences in the metabolite pattern between caries active and caries inactive children. For the basic research LC-MS/MS<sub>MRM</sub>-methods were developed to identify and quantify key-metabolites like amino acids, short chain fatty acids and carbohydrates in saliva and pellicle. Furthermore a comprehensive untargeted UPLC-TOF-MS-method for metabolic profiling was developed as the basis for the prediction of caries activity and the identification of marker substances according to caries disease. Therefore, saliva and pellicle samples of about 60 children with different clinically characterized states of caries were analyzed by means of UPLC-TOF-MS. By using multivariate data analysis models for caries activity were build arranging the probands in caries free and caries active groups and supporting the prediction of the caries state or the susceptibility for caries.

Metabolomic profiling of saliva and pellicle samples collected from healthy and caries patients

255th National Meeting (2018)

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