Carbohydrates are structurally diverse and functionally important biomolecules. Many naturally occurring carbohydrates contain modifications that are added after the formation of the glycosidic linkage, named as post-glycosylational modifications (PGMs). We have developed highly efficient glycosyltransferase-based one-pot multienzyme (OPME) systems for synthesizing structurally complex naturally occurring carbohydrates and their non-natural derivatives. In these systems, desired modifications are introduced at the monosaccharide building blocks by chemically synthesis. Substrate promiscuous enzymes are then used in one-pot for activation and transfer of the modified monosaccharides to suitable acceptors for glycosyltransferases. The OPME systems can be used in sequential for building up longer and complex structures. The straight-forward synthetic schemes can be combined with facile purification processes for efficient production of desired compounds. Sialidase inhibitors can also be synthesized by novel OPME systems from simple monosaccharides and derivatives. Our recent progess on the development of the OPME strategy will be presented.

One-pot multienzyme (OPME) chemoenzymatic synthesis of carbohydrates and sialidase inhibitors

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.