Developed an efficient/economical process to the synthesis of Poly-L-Ornithine.HCl with narrow molecular weight range. The general procedure to synthesize Poly-L-Ornithine.HCl was to polymerize N-carboxyanhydride of N<sup>δ</sup>-CBZ-L-Ornithine to make the protected polymer and remove CBZ protecting group from the protected polymer with HBr/acetic acid, dialyze the solution and lyophilize to isolate Poly-L-Ornithine.HBr. Then dissolve the Poly-L-Ornithine.HBr in 2M Hydrochloric acid, and dialyze again to remove the excess Hydrochloric acid and lyophilize to isolate the final product Poly-L-Ornithine.HCl, which results invariably much lower and unpredictable yield (≤ 50%). The new process reduced the most energy consuming step, lyophilization by 50% by making the salt exchange without isolating Poly-L-Ornithine.HBr. Also, the new greener scalable process decreased the cycle time by 30%, controlled the molecular weight variation, decreased the manufacturing cost, decreased the use of purified water by &gt;90% by switching from dialysis to ultrafiltration and also increased the yield without compromising quality. Both purification methods, dialysis, and ultrafiltration gave similar MW profile. This polymer is used in drug delivery applications.<br/>

Efficient synthesis of polyamino acid polymers

255th National Meeting (2018)

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