Simple, accurate, low-cost, biosensor arrays for clinical measurements of biomarker proteins for early detection and monitoring of cancer are critically important. Successful development of such inexpensive devices will lead to reliable on-the-spot cancer diagnosis, improved therapeutic outcomes at lower costs, decreased patient stress, and new targeted therapies. Herein, we report on a simple microfluidic-based immunosensor array coupled with PEG modified multi-labeled magnetic beads for high sensitivity multiplex electrochemical detection of interleukin-6 and interleukin-22 biomarker proteins in calf serum. Elevated levels of IL-6 and IL-22 in patients indicate that the patient may have various types of cancer, such as Cutaneous T-cell lymphoma (CTCL), a form of cancer that primarily affects the skin. Capture antibodies were immobilized onto nanostructured 8-electrode array followed by binding a PEG modified magnetic bead bioconjugate with off-line captured antigen (HRP/MB/Ab2-Ag)-PEG in a microfluidic device. The electrical signal in the sandwich assay was generated by injection of a mixture of H2O2 and hydroquinone charge mediator. Upon optimization of the experimental variables affecting the immunosensor performance, the immunosensor provided linear ranges between 50 fg/mL and 1000 fg/mL for both IL-6 and IL-22, these range being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 50 fg/mL for both IL-22 and IL-6 in calf serum. Results show great promise of a point-of-care diagnosis system for simple early detection of CTCL.

Electrochemical immunosensor for simultaneous determination of IL-6 and IL-22 in serum using nanostructured electrode array coupled to PEG-multilabeled magnetic particle and a microfluidic device

255th National Meeting (2018)

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