It has been difficult to explore the biological properties of specific glycans in the context of cells. We are addressing this deficiency by developing a cell-surface engineering strategy based on the use of a CMP-Neu5Ac derivate that is modified at C-5 by a bi-functional entity composed of a complex oligosaccharide and biotin. It was found that recombinant ST6GAL1 can readily transfer the modified sialic acid to glycoprotein acceptors of living cells resulting long-lived display. It made it possible to display a range of well-defined oligosaccharides, such heparan sulfate and matriglycan, on the surface of cells that do not present these oligosaccharides resulting in a gain of biological activity. The new approach makes it possible to establish structure-function relationships in the context of cells.<br/>

Cell-surface glyco-engineering using sialyl transferases and modified CMP-Neu5Ac derivatives

255th National Meeting (2018)

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