This work is to face the challenge in current clinical setting to monitor small molecule drugs accurately and rapidly for the point-of-care (POC) diagnosis. Acetaminophen (AP), which is a commonly used OTC analgesic drug, its overdose has been determined as the major cause of acute liver failure in the US and UK. However, there is no rapid and accurate detection method available for the emergency room. This AP sensing strategy relies on the previously unexplored strong interaction between the AP and arginine (Arg) molecule we found that, surprisingly, as many as 4 hydrogen bonds can be formed between one Arg molecule and one AP molecule. By taking advantages of this structural selectivity and high tenability of hydrogen bonding, arginine, which immobilized on the graphene surface via electrostatic interaction, was utilized to structurally capture AP molecule and quantified by EC techniques for sensor use. Interestingly, bonded AP still remained the perfect electrochemical activities, which was used as the quantification technique. To verify the feasibility of this novel assay, based on multi hydrogen bonds manipulated single-molecule recognization (eMuHSiR), the both pharmaceutical and serum sample were examined. In the commercial tablet measurement, no significant difference of the eMuHSiR results was seen with the standard methods. For measuring the AP concentration in the mice blood, the substances in serum, such as sugars and fats, would not bring any interferences to the eMuHSiR in a wide concentration range. This eMuHSiR method open the way for future development of numerous other types of small molecule detection for the POC testing.

Point-of-care determining acetaminophen with multi hydrogen bonds manipulated single-molecule recognization

255th National Meeting (2018)

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