Peroxidase is an enzyme found ubiquitously throughout nature, important for its role in controlling hydrogen peroxide (H2O2) levels within the cell by performing oxidation reduction reactions using H2O2 as a primary substrate. In this work, peroxidase from Taraxacum officinale, colloquially known as the common dandelion, was investigated. Peroxidase was isolated and extracted from three separate parts of the plant: the stem, root, and leaves. Using pyrogallol, o-phenylenediamine (OPD), or guaiacol as a secondary substrate, the enzyme’s kinetic parameters were determined via spectrophotometry due to the visible color change associated with the reactions. The differing kinetic parameters for enzyme isolated from different parts of the plant indicates the presence of isozymes between the plant tissues, which are currently under investigation.

Isolation and characterization of dandelion (Taraxacum officinale) peroxidase

255th National Meeting (2018)

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