We report a convenient and versatile strategy for surface-grafted glycopolymer constructs with the goal of surface modification that controls the orientation and grafting density of carbohydrate sidechains. This approach employs a difunctional hydrazine linker, chemically modified to an active ester containing poly(pentafluorophenyl acrylate) grafted scaffold, to conjugate a variety of saccharides through their reducing end. The successive conjugation steps are carried out under mild conditions and yield high surface densities of sugars, as high as 4.8 nmol/cm2, with an intact structure and retained bioactivity. We demonstrate this glycosylated surface can bind specific lectins according to the structure of its pendant carbohydrate. We also use this approach to fabricate sialylated glycosurfaces to study the biological events of a human respiratory tract pathogen, Mycoplasma pneumoniae.

Versatile glycosurfaces fabrication on poly(pentafluorophenyl acrylate) grafted scaffold via hydrazide conjugation and Its application in Mycoplasma Pneumoniae

255th National Meeting (2018)

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