Perfusion technology has been successfully used for the commercial production of biotherapeutics, in particular unstable recombinant proteins, for more than a decade. However, there has been a general lack of high-throughput cell culture tools specifically for perfusion-based cell culture processes. Here we have developed a high-throughput cell retention operation for use with the ambr® 15 bioreactor system.  Experiments were run in both 24 and 48 reactor configurations for use in media development, clone screening and an assessment of perfusion mimic model variations.  Employing an offline process for cell retention and a variable volume model developed with Matlab computational software, the established platform has demonstrated cell culture performance (&gt; 90 E6 cells/mL and &gt;95% viability) and productivity comparable to bench scale bioreactors. The automated, single use, high-throughput platform is a powerful tool that enables us to have rapid and efficient process development of perfusion-based cell culture processes.<br/>

Development of a novel, high-throughput platform for efficient perfusion-based cell culture process development

255th National Meeting (2018)

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