High temperatures encourage the rapid growth of polymersomes composed of amphiphilic diblock copolymers on cellulose paper. Use of high temperatures, however, could preclude the encapsulation of temperature labile cargo such as proteins and other biological molecules if the cargo is loaded simultaneously to the process of growth — the current convention for producing protein loaded polymersomes. This poster reports a process that can produce tens of thousands of protein-loaded PBD46PEO30 polymersomes under mild conditions with a modification that temporally decouples the process of growth of polymersomes from the process of loading cargo into the polymersomes on cellulose paper. Encapsulation was efficient. Only approximately 20 percent of the polymersomes had intensities in the lower 10 % of the distribution. The simple diffusion controlled bench top batch process resulted in ~ 20,000 polymersomes within about 2 hours from 5 mm2 piece of filter paper.

Temporally decoupled growth and loading of a protein cargo into polymersomes using cellulose paper

255th National Meeting (2018)

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