High throughput and low usage of liquid and stationary phases has resulted in the wide use of Simulated Moving Bed (SMB) chromatography in the chemical, food, and pharmaceutical industry, e.g., to produce high-fructose corn syrup and pharmaceutical enantiomers. In the bioprocess area, it has also been demonstrated for the capture chromatography of monoclonal antibodies. However, its application to the polishing chromatography steps of biomacromolecules, in which resolution is essential, has been extremely limited. Polishing chromatography requires gradient or sequential stepwise elution to cope with the ‘all or nothing’ adsorption behaviour of macromolecules. These changes in salt (or other modulator) complicate the design process of a polishing SMB considerably. In this poster, two simple methods of achieving a fully-continuous ternary stepwise-elution SMB separation are contrasted. These two approaches to ternary separations are experimentally demonstrated for the centre-cut separation of ovalbumin variants. This extremely difficult separation is a good representation of product variants in industrially relevant separations. Furthermore, simulation data is presented to highlight the relative advantages and disadvantages of the two approaches to centre-cut stepwise elution SMB.

Centre-cut separation of biomolecules by stepwise-elution SMB

255th National Meeting (2018)

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