Multiple studies have proven that the protein Obg-like ATPase 1, referred to as OLA1, is associated with different types of cancers. This protein has important functions in cellular processes and plays a significant role in the migration and invasion of breast cancer cells. hOLA1 is predicted to be a targeted substrate for α N-terminal RCC1 methyltransferase (NRMT1) because it shares the N-terminal Met-Pro-Pro-Lys motif sequence. NRMT1 can catalyze the N-terminal methylation by the addition of S-adenosyl-L-methionine (SAM), a methyl donor. This project aims to study whether hOLA1 can be N-terminally methylated by NRMT1 as well as its roles in carcinogenesis. The preliminary studies focus on the in vitro methylation at a peptide level because it is more cost effective and easier to characterize. The N-terminally methylated peptide was characterized by using the techniques of Mass Spectrometry and HPLC. These results indicate that the protein hOLA1 can be methylated by NRMT1 in vivo.

The preliminary study of hOLA1 N-terminal methylation catalyzed by NRMT1

255th National Meeting (2018)

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