Purification of viruses can take advantage of their large size compared to most contaminants of interest. A novel chromatographic technique for virus purification is supported by media such as Capto Core 700<sup>TM</sup>, which combines an inert size-selective surface shell with an exclusion limit of 700 kDa, which excludes viruses, with a multimodal octylamine ligand core which captures most proteins. In this work, we have adapted this exclusion technology to virus detection and demonstrated the method using M13 bacteriophage as a model virus. We added enzyme-labeled target-specific antibodies to samples with/without M13 and separated the enzyme-labeled M13 particles on a Capto column. In the absence of M13 target, the antibody-enzyme conjugate is small enough to enter the core and be adsorbed to undetectable levels. In the presence of M13, antibody-enzyme conjugate bound to the virus is excluded from adsorption and is detected in the eluate.

Virus detection using restricted-access adsorbents

255th National Meeting (2018)

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