The interactions between DNA and various transition-metal complexes were characterized using Surface-Enhanced Raman Spectroscopy (SERS). Using gold nanoparticles (AuNPs) as the signal enhancer, shifts and intensity changes in the vibrational peaks of the metal complexes were used to characterize the interactions. Water-soluble zinc and nickel metal complexes with different ligands in octahedral or square planar geometries were used. Various quadruplex sequences, including the thrombin-binding aptamer (15mer) and human telomeric DNA (22mer) were then added to these solutions at different concentrations to characterize the types of interactions the DNA has with the metal complexes. Previous studies showed that the DNA and metal complexes interacted, and changes in vibrational peak intensity and position of the metal complexes were used to determine the nature of the interaction. The goal of this study is to develop another method of biodetection for DNA using these metal complexes.

Study of the Interactions between DNA and Metal Complexes using Surface-Enhanced Raman Spectroscopy

255th National Meeting (2018)

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