This talk will describe an approach for using mass spectrometry to analyze peptide arrays. The arrays are prepared by immobilizing peptides to self-assembled monolayers of alkanethiolates on gold. This arrays are then treated with reactants—either chemical reagents or enzymes—and then analyzed using the SAMDI technique to identify the masses of substituted alkanethiolates in the monolayer and therefore a broad range of reactivities and post-translational modifications—including kinase, protease, methyltransferase and carbohydrate-directed modifications—and for discovering chemical reactions. This talk will describe applications to high throughput experiments, including the discovery of reactions, the preparation of peptide arrays to profile the enzyme activities in cell lysates and identification of peptide tags that can be efficiently glycosylated. These examples illustrate the broad capability of the SAMDI method to profile and discover molecular activities in the molecular sciences.

Combining peptide arrays and mass spectrometry for high throughput experiments

255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.