255th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

American Chemical Society

Upstream Processes

Getting more for less: Leveraging epigenetics to increase process yield

New fungal RiPP family changes the rules for modified peptide biosynthesis

Engineering synthetic consortia inspired by the rumen microbiome

Technical capability of Raman spectroscopy for in-line quantification of antibody product quality

Operon refactoring and construction assistant (ORCA): An integrated workflow to refactoring biosynthetic gene clusters

Development of a statistical model to support perfusion bioreactor scale-up

A synthetic biology approach to precursor-directed biosynthesis of complex polyketides and terpenes

Understanding glycosylation variability in CHO cell cultures

Expression and in vitro reconstitution of novel lasso peptide gene clusters

Redefining polyketide synthase modules

Transcriptional-sensor based increase in peroxisomal fatty acyl-CoA flux improves fatty alcohol production in Yarrowia lipolytica

Development and implementation of perfusion N-1 for mammalian cell culture to enhance process productivity

Engineering biosynthesis of the anticancer alkaloid noscapine in yeast

Optimizing the addition antifoam for process intensification: A case study using small scale models

Media formulation optimization based on multi-scale modeling of heterogeneity in mammalian cell culture process

RNA-seq data reveals metabolic regulation in Chinese hamster ovary cell culture

Development of high-producing CHO cell lines through target-designed strategy

A novel high density N-1 batch seed strategy development for CHO cell culture manufacturing

Synthetic biology tools for the purple, non-sulfur bacterium Rhodopseudomonas palustris CGA009

Engineering uniform-shear rate bioreactors to mimic bone marrow and lung vasculature niches for the production of platelets ex vivo

Computational fluid dynamic modeling and design of a novel single-use airlift perfusion reactor prototype for human cells

MODCELL: A prototype for modular cell engineering

Understanding lipogeneity in oleaginous yeast: source of reducing equivalents for proliferating cell and non-proliferating cell

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Interrogation of critical metabolic pathways important for compartment-specific redox homeostasis in cancer cells

Electro-active communities for hydrogen production from biomass streams

Investing in process development for MSC production in stirred tank bioreactors

Overcoming cell line limitation and improving medium in a perfusion process for manufacture of a labile and difficult-to-express enzyme

Understanding the effect of preparation methods on bioavailability of components in a cell culture feed

Optimization, evolution, and control of pathways and metabolism in yeasts

Microbiome-virome interactions in bovine rumen: Elucidating metabolic transactions through metabolic modeling

Exploring non-natural pathways and native efflux systems for improved aromatic chemical production in Escherichia coli

Engineering modular microbial communities for cellulose utilization and bioproduct synthesis

Global regulation of lignocellulolytic proteins from anaerobic fungi in response to substrate lignin content and composition

Designing a continuous perfusion process for manufacturing antimicrobial peptides in Drosophila melanogaster S2 cells

MinGenome: Top-down synthesis of genome minimized strains for bioproduction

Use of an Escherichia coli pyruvate-overproducing platform strain to produce L-valine

Production and purification of oncolytic measles viruses

Evaluation of a cyanobacterial consortium for efficient polysaccharide production

Media optimization towards a high-titer platform process

Optimization of heterologous mevalonate production in E. coli with a combinatorial promoter library and novel biosensor

Expression of snake-antivenom peptide in Pichia pastoris

Building a bridge between cell-free experimentation and cellular metabolic engineering

Metabolic modeling of microbial communities

Leveraging –omics techniques to enhance productivity of biologics in microbes

Elucidating amino acid metabolism in CHO cells

Cell characterization using a novel online cell quantitation microscopic technology in comparison with conventional offline methodologies

Enabling natural products discovery from anaerobic fungi via integrated ‘omics’ approaches

Engineering species-like barriers to sexual reproduction for applications in public health, agriculture, and the environment

Maintaining product quality from early to late stage upstream process development

Impact of high extracellular lactate on induced pluripotent stem cell metabolism

Advancing mammalian metabolic engineering through kinetic model optimization

Improving global access to biotherapeutics

Engineering gut fungal membrane-embedded transporters for metabolic engineering in Saccharomyces cerevisiae

Sustainable production of industrially relevant biomonomers: A photosynthetic microbial consortia approach

Limitations of subcloning as a tool to characterize homogeneity of a cell population

Genomic scale metabolic network reconstruction of Nostoc sp. PCC 7120 for the analysis of cyanophycin production

Microdroplet-assisted evolution of Yarrowia lipolytica for the production of secreted riboflavin (vitamin B2)

Discovery of metabolic pathways for lipid-production from lignin-derived phenolics in a non-model oleaginous yeast

Unravelling molecular mechanisms that govern the shift to a lactate consumption metabolic state in CHO cell fed-batch cultures

From genotype to fluxome to a comprehensive kinetic model of Escherichia coli

13C metabolic flux analysis in microbial communities: An integrated multi-scale modeling approach

Utilization of computational fluid dynamics (CFD) to guide scale up of cell culture bioreactors

Nature as the ultimate combinatorial biosynthetic chemist: Inspiration for engineering natural products biosynthesis

Quantitative multi-dimensional single-cell analysis of autophagy during myogenesis

Tailoring chain-length specificity for oleochemical production in bacteria

Understanding product sieving challenges in TFF perfusion cell culture and developing strategies for mitigation

Assessment of the genomic instability of CHO cells for cell line development

Global metabolic rewiring for 2,3-butanediol production in cyanobacteria

Elucidating core design principles to engineer nonconventional yeasts as novel microbial factories

What cells want vs. what cells need: Simplification of chemically defined media for optimal performance

Mapping the energy profile of cell culture through advanced lipidomics and metabolomics

Continuous processing: Lessons learned from the past and strategies for success in the future

Process improvement of a high density fed-batch process using N-1 perfusion seed cultures

Investigating the global landscape of chromatin accessibility to improve production Chinese hamster ovary (CHO) cell line stability

Developing the thermotolerant yeast Kluyveromyces marxianus as a microbial host for volatile ester biosynthesis

Polysaccharide chemical structure controls on fermentation by gut microbiota, metabolic outputs, and interspecies interactions

Upstream Processes

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Getting more for less: Leveraging epigenetics to increase process yield