Carbohydrates play an important role in a variety of biological processes such as cell signaling, migration and cancer cell metastasis. Neutral and low molecular weight monosaccharides have recently become an alternative surface modifier for bioconjugation of plasmonic nanoparticles to the widely used bulky long chained poly(ethylene glycol) (PEG) due to their low cytotoxicity, stability and active targeting ability. In this study, we achieved the biofunctionalization of 4-aminophenyl β-D-glucopyranoside (Glu) and/or 4-aminophenyl β-D-galactopyranoside (Gal) onto 40 nm plasmonic gold nanospheres (AuNSs) through EDC/NHS chemistry. The nanometer precision non-fluorescent single particle tracking (SPT) of the monosaccharides modified AuNSs on both synthetic lipid membranes and live cell membranes using correlation mapping algorithm in differential interference contrast (DIC) microscopy was demonstrated. Although the subtle difference on the monosaccharide structures result in similar lateral diffusion of the monosaccharides modified AuNSs on membranes, a dramatic difference on the internalization kinetics and efficiency was observed in live cell. Cholesterol level modulations elucidate the effects upon the changes of the cholesterol content in the membranes on the cellular uptake of the monosaccharides modified AuNSs, thus lead to the better understanding of the delivery efficiency of nanomedicines.

High-precision non-fluorescent single particle tracking of monosaccharide conjugated gold nanoparticles on membranes

256th National Meeting (2018)

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