Cutaneous wound healing is an intricate process whereby re-epithelialization and fibroplasia initiates after injury. We investigated the effects of four kinds of beta-glucan, which are derived from barley, yeast, mushroom, and euglena, on th e migration and viability of keratinocyte or fibroblast using the in vitro scratch wound healing assay, invasion assay and MTT assay as well as in vivo assay. All kinds of beta-glucan showed significant increase on keratinocyte migratory effect at 20μ M and no toxicity on dermal fibroblast. Especially treatment of beta-glucan derived from mushroom (Schizophyllum commune) on keratinocyte cells promoted in vivo wound closure. The FAK/Src pathway has well known to affect cell migration by forming lamellipodia. It also promotes phosphorylation of FAK/Src in time-dependent. ROS is associated with fibroblast differentiation to contract dermal layer and synthesize collagens. We found that fibroblast was activated by increasing NOX expression. We propose that beta-glucan derived from mushroom is capable of promoting keratinocyte migration via the induction of FAK/Src phosphorylation resulting acceleration of wound closure and activating dermal fibroblast differentiation through NADPH oxidase for matrix remodeling.

Wound healing effects of β-glucan

256th National Meeting (2018)

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