Due to the increasing concerns on the environment, the market demands for bio-based chemicals are dramatically increasing. Glutaric acid, an intermediate in amino acid pathways in cell, is becoming a promising C5 diacid chemical as a monomer for polymer and a plasticizer to substitute phthalate plasticizers. Currently, glutaric acid can be produced using butyrolactone with potassium cyanide which is highly toxic. Since microorganisms have been used as the biocatalysts to produce various chemicals to replace the materials from petrochemical industry, the biological conversion of 5-aminovaleric acid into glutaric acid was studied by using 4-aminovalerate aminotransferase or 5-aminovalerate aminotransferase (gabT or davT) and succinate semialdehyde dehydrogenase or glutarate semialdehyde dehydrogenase (gabD or davD). Recombinant Escherichia coli strains expressing gabTD genes or davTD genes were developed to convert 5-AVA into glutaric acid. The genes from various strains were examined and the optimum pH condition was determined. Furthermore, the effects of cofactors such as NAD and PLP were studied to increase the conversion rate of 5-AVA.

Glutaric acid production from 5-aminovaleric acid using aminovalerate aminotransferase and semialdehyde dehydrogenase in Escherichia coli as whole cell biocatalyst

256th National Meeting (2018)

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