In this presentation, we will demonstrate that LFIA strips integrated with H-Pt NPs could detect analytes within 15 min by easy operation and sensitivity ~20 times higher than that of conventional LFIA strips. In addition, by processing digital images of LFIA strips acquired by a smart cellular phone, which is being ubiquitously available, quantification of analytes could be performed. Considering that H-Pt NPs stored in harsh environments maintained peroxidase-like activity, the H-Pt NP-loaded LFIA strips developed in this study will be useful for many POC bioassay applications. Fe<sub>3</sub>O<sub>4</sub>-Pt/Core-Shell nanoparticles (MPt/CS NPs) with various compositions were synthesized. Enrichment of liquid samples was achieved by magnetic properties of MPt/CS NPs, whereas catalytic properties of MPt/CS NPs allow signal amplification by enzyme-like reactions. By integrating MPt/CS NPs in lateral flow immunoassay strips, one of the widely used point-of-care bioassay devices, and harnessing magnetic and enzyme-like properties of MPt/CS NPs, two orders of magnitude increase in sensitivity was achieved compared to conventional lateral flow immunoassay based on Au nanoparticles.

Point of care bioassay system based on enzyme-mimetic nanomaterials

256th National Meeting (2018)

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