A facile enzyme immobilization strategy with hierarchically porous biomimetic metal–organic frameworks (MOFs) was established to prepare artificial enzyme systems for cascade reactions. The MOF HP-PCN-224(Fe) with peroxidase-like activity and tunable hierarchical porosity was synthesized via a modulator-induced strategy. Glucose oxidase (GOx) and uricase could be immobilized efficiently on the HP-PCN-224(Fe) with very high load capacity and excellent stability. Two colorimetric sensors for the detection of glucose and uric acid (UA) were realized through the cascade reactions catalyzed by the mimic multi-enzyme systems GOx@HP-PCN-224(Fe) and Uricase@HP-PCN-224(Fe). This work displays the great potential to construct highly functional biocatalysts by integrating the merits of both natural enzymes and MOF mimics, which are promising for applications in biosensing and biomimetic catalysis.

Rational design of mimic multi-enzyme systems in hierarchically porous biomimetic metal-organic frameworks

256th National Meeting (2018)

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