Recent trend for fluorescence detected analytical applications is the use of longer wavelength fluorophores and encapsulation of fluorophores by utilizing nanoparticles. Long wavelength fluorescence in analytical chemistry has several advantages such as lower background interference especially in biological matrices. While many different types of nanoparticles have been utilized for analytical applications, silica nanoparticles have significant advantages in biological systems. Silica nanoparticle synthesis using copolymerization of fluorescent dyes an easily controllable process by using modified TEOS reactive analogues. The fluorescence intensity of silica nanoparticles can significantly be increased by co-polymerizing a large number of dye molecules. Using NIR dyes are excellent choices for high sensitivity measurements but one major disadvantage is that most fluorescence instruments for bioanalytical applications are designed for shorter excitation wavelengths frequently matching the optical properties of fluorescein, a widely used fluorophore. One approach to make these new fluorescent silica nanoparticles more compatible with this excitation wavelength range is the development of fluorescent silica nanoparticles utilizing fluorescence energy transfer. This presentation discusses the facile synthesis and analytical applications of such silica nanoparticles containing copolymerized single or multiple fluorophores. The surface properties of fluorescence silica nanoparticles can be modified by covalently binding hydrophobic or hydrophilic molecules on the surface to achieve biocompatibility. Biocompatibility was evaluated by hemolytic experiments. Typical applications of these particles are for immunochemistry, flow cytometry, CE, forensic applications, etc. Modifying surface properties of silica nanoparticles enhances their analytical utility.<br/>

Fluorescent copolymerized silica nanoparticles: Synthesis and applications

256th National Meeting (2018)

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