A programmable pre-assembly method is used to produce near-infrared fluorescent molecular probes with tunable multivalent binding properties. The modular assembly process threads one or two copies of a tetralactam macrocycle onto a fluorescent PEGylated squaraine scaffold containing a complementary number of docking stations. Appended to the macrocycle periphery are multiple copies of a ligand that is known to target a biomarker. The threaded complexes are effective multivalent molecular probes for fluorescence microscopy and in vivo fluorescence imaging of living subjects. The pre-assembly method is extremely versatile and can be used in a parallel combinatorial manner to produce libraries of near-infrared fluorescent multivalent molecular probes for different types of imaging and diagnostic applications, with incremental structural changes in the number of targeting groups, linker lengths, and degree of PEGylation.<br/>

Pre-assembly of near-infrared fluorescent multivalent molecular probes for biological imaging

256th National Meeting (2018)

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