Hydrogen peroxide (H2O2) plays a critical role in the regulation of multifarious physiological processes. We developed a sensor containing a mercaptopropionic acid (MPA) monolayer covalently immobilized with Horseradish peroxidase (HRP) enzyme for the electrochemical detection of hydrogen peroxide (H2O2). A gold foil substrate was chemically treated with nitric acid and were used as working electrode. Platinum wire and Ag-Ag/Cl were used as counter and reference electrodes, respectively. The acid treated gold electrode with the immobilized enzyme shown to have improved catalytic activity in the reduction of H2O2. The steady-state current response increases linearly with H2O2 concentration from 100 nM to 9 mM with a low detection limit of 60 μM and showed a sensitivity of 0.4 mA/ mM cm2. This electrochemical sensor is demonstrated to be highly selective and sensitive in the presence of interfering analytes. The improved activity and simple preparation method of the electrode makes the MPA-HRP modified gold electrode promising for being developed as an attractive robust material for electrochemical H2O2 sensing.

Nanoporous gold-based biosensor for the determination of hydrogen peroxide

256th National Meeting (2018)

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