The complexity of biological cellular systems is a new frontier and challenge to analytical chemistry. Simultaneous analysis of multiple cell types requires identification of such types (genotyping) and measurement of the properties of interest. Mass cytometry is a technique capable of measuring up to 50 parameters per cell in a high throughput fashion. The technique relies on the use of antibodies bearing a lanthanide isotopes, specific to each antibody. The approach has found widely spread use in immunology and cancer. Unfortunately, the analytical power of such technology has been compromised due to non-specific binding, the definition of factors that compromise reproducibility and the lack of expansion to new biological systems. This presentation will provide an update on our efforts to make this technique analytically reliable. We provide a road map on how to correct for non-specific binding using isotypes and normalization using markers associated with cellular mass. We will also demonstrate the use of this technology to monitor alterations in autophagy (cellular self-eating), which is a cellular function significantly affected in multiple diseases.

Mass cytometric analysis of single-cell cells

256th National Meeting (2018)

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