Our lab has recently developed mass spectrometry imaging workflows for N-glycans and other glycan classes in multiple types of tumor tissues. Glycoproteins account for the majority of the proteins located at the cell surface and in the extracellular environment.  During carcinogenesis the normal function of these glycoproteins as binding ligands for cell-cell adhesion, extracellular matrix molecules, signaling receptors, and immune cells becomes altered. Our approach to evaluate these complex glycan interactions in normal and tumor tissues has been to use high resolution MALDI-FTICR and ion-mobility mass spectrometry imaging. The basic workflow has led to development of related approaches for direct analysis of constituent N-linked glycans on individual glycoproteins. Example data and workflows for how we have combined mass spectrometry imaging workflows for biomarker discovery and glycoprotein identification will be presented.<br/>

Mass spectrometry imaging of N-glycans in cancer tissues

256th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.