Lipid translocation or flip-flop is an essential process in cells to maintain membrane asymmetry by transporting newly synthesized lipids for their proper location. Previous investigations of lipid translocation have relied on spin- or fluorescently-labeled lipids but these modifications can alter the kinetics of flip-flop. To accurately convey the kinetics and thermodynamics of native lipid motion, a label-free approach was applied. Our results suggest that the rate of flip-flop is much faster compared to reported results that used spin- or fluorescent labeling.

Lipid flip-flop measured by sum frequency vibrational spectroscopy

256th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.