Layer-by-layer (LbL) coated microneedle (MN) is a promising approach for transdermal drug delivery. One drawback of all state-of-the-art LbL MN strategies is the long epidermal application time (15-90 min) required for the drug coatings to detach from MN surface, which severely diminishes the attractiveness of such therapies. Here we developed a new release platform based on charge-invertible micelles requiring only 1-2 min skin insertion time to implant LbL film in vivo, faster than any other current method. Utilizing our new platform, we successfully implanted LbL film containing ovalbumin (OVA) antigen on mouse ear and achieved sustained release over 4 days. This has led to robust immune response compared to same amount of OVA injected subcutaneously and intramuscularly, as demonstrated by significantly higher anti-OVA IgG1 antibody level in mouse serum. This work provides an easy and rapid LbL film implantation strategy for microneedle-based vaccinations.

Synthetic charge-invertible micelles for rapid and complete implantation of LbL drug films coated on microneedle patches for enhanced transdermal vaccination

256th National Meeting (2018)

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