Preliminary screening of the crude methanolic extracts of seeds of Cassia occidentalis for phytochemicals, elemental constituents,antimicrobial and acute toxicity (LD50),revealed the presence of flavonoids, steroids, terpenes, fatty acids, resins, glycosides, tannins and anthraquinones. Zinc, selenium, iron, cadmium and chromium were also found in appreciable quantities. The extracts were not toxic even at 5000m/kg. Extraction with ethylacetate followed by isolation and purification using series of chromatographic techniques yielded two pure compounds with melting points of 190-193 and 206-208oC which were identified using UV, FTIR, MS, one and two Dimensional 1HNMR and 13CNMR as 1,1`,8,8`-tetrahydroxy-6,6`-methoxy-3,3`-dimethyl-10,10`bianthracene-9,9`dione and 1, 8-dihydroxy-3-methoxy-6-methylanthraquinone. The compound1,8-dihydroxy-3-methoxy 6-methyl anthraquinone showed strong activity against Trypanonsomebruceibrucei a causative organism for African Trypanosonomiasis.

Extraction and characterisation of an anti trypanonsomal compound from the seeds of Cassia occidentalis

256th National Meeting (2018)

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