Novel materials fabricated via self-assembly of biological molecules need to be both functional as well as biocompatible. Nanotubes composed of aromatic dipeptides dityrosine and tryptophan-tyrosine have been synthesized via solution-phase self-assembly (SPSA). Differential scanning calorimetry and thermogravimetric analysis were used to analyze the thermal behavior, whereas Fourier transform infrared spectroscopy, liquid chromatography-mass spectroscopy, and Raman scattering, and circular dichroism spectroscopy, and powder x-ray diffraction were used to analyze the chemical composition and secondary structure. Additionally, computational studies provided the mechanisms of interactions involved in self-assembly(Figure.1). Also, confocal and scanning electron microscopes(SEM) were used to study the morphological features of these nanotubes.<br/><br/>The cellular interactions and physiological effects of these SFSA nanotubes were examined using confocal microscopy, SEM, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and <i>in vitro </i>dopamine-enzyme linked immunosorbent assays on the rat adrenal pheochromocytoma cells (PC-12)(Figure.1), human cell-lines of M17 and SH-SY5Y. In order to understand their influence on dopamine and neural differentiation genes using real-time polymerase chain reaction (RT-PCR). Similar studies with chemical vapor deposited nanotubes were performed to study the effects of nanotube scaffolds, especially neural differentiation.

Synthesis and characterization of self-assembled peptide nanotubes: Scaffolds for neural cell differentiation

256th National Meeting (2018)

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