Nomilin, a predominant lipophilic molecule present in citrus seeds (agro-food industrial byproduct), seems to have protective effects in chronic disease prevention and reducing metabolic disorders. In the present study, nano-based delivery system was formulated and characterized to overcome the solubility of bioactive nomilin. The effect of poly (lactic-co-glycolic acid) (PLGA) resomers with varying copolymer composition and molecular weight on nomilin for entrapment, yield and release characteristics were studied. Spherical shaped nomilin loaded PLGA nanoparticles were prepared with didodecyl dimethyl ammonium bromide (DMAB) and pluronic F-68 (PF-68) at the optimized effective micellar concentration of 1% and 3% respectively at 30%, 40% and 50% nomilin loading through modified emulsion diffusion evaporation technique. Relatively smaller nanoparticles (135 nm to 150 nm) were obtained from all PLGA resomers with DMAB as surfactant. Nanoparticles prepared from PLGA polymers with both the surfactants resulted in narrow particle size distribution. Among the different resomers, PLGA (50:50) polymer with PF-68 surfactant at 40% nomilin loading recorded the maximum nomilin entrapment efficiency of 77.2% and only 50.5% of entrapped nomilin was released in the acetate buffer over 96h study exhibiting slow release rate pattern. PLGA (50:50) polymer and PF-68 surfactant combination at all three levels of nomilin loading obtained higher nanoparticle yield of 80% to 90%. Significantly higher inhibition of a-amylase and angiostensin converting enzyme (ACE) activity were observed with nomilin nanoparticles prepared from PF-68 and DMAB, respectively in comparison to nomilin suggesting the potential role of nomilin to improve human health.

Nomilin nanoparticles enhances the inhibition of α-amylase and angiotensin-converting-enzyme

256th National Meeting (2018)

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