The preferred strategy for the production of natural products and mammalian/ environmental fate metabolites is chemical synthesis. However, total synthesis is not always a viable option due to the structural complexity of natural products and many metabolites. Microbial fermentation and biotransformation are established routes to access unlabelled natural products and metabolites, as alternatives to chemical synthesis. Combining microbial fermentation and biotransformation processes with radiochemistry enables the development of microbial production methods which can then be transferred to radiochemistry facilities for optimum production using radiolabelled material. Using case studies, this presentation will describe strategies for the microbial production of [14C] labelled complex natural products via labelled precursor feeding, as well as the production of metabolites of [14C] labelled small molecule compounds.

Production of isotopically labelled natural products and metabolites by microbial fermentation and biotransformation

256th National Meeting (2018)

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