Hypertension is a progressive cardiovascular syndrome caused by many factors. The public research indicates that consumption of tea prevent hypertension. Tea is rich in polyphenols, which catechins constitute approximate 80% of the total amount of polyphenols. Recently, some methylated catechins have been found and isolated. The methylated catechins are relatively stable, and showed anti-allergy effects. However, little is known for its vasodilation effect. The current study investigates the vasodilation function of 3" methylated ECG as well as ECG and EGCG by using rat mesenteric artery.<br/>     Our results revealed that all three catechins have vasodilation effect and this effect shows a concentration dependent manner. Using norepinephrine induced contraction of the second branch of mesenteric artery, 20μM and 40μM 3" methylated ECG significantly decrease the contraction of mesenteric artery compared to ECG. Moreover the relaxation effect of 3" methylated ECG on the artery is similar to the EGCG. Mechanism study showed that the three catechins inhibit angiotensin II induced phosphorylation of protein kinase C-dependent phosphatase inhibitor of 17kDa (CPI-17) in cultured rat vascular smooth muscle cells, which cause vaso-contraction. Furthermore, the three catechins also inhibit norepinephrine induced phosphorylation of CPI-17 in rat aorta. Therefore, the effect of vascular relaxation of 3" methylated ECG, ECG and EGCG may achieved by inhibiting the phosphorylation of CPI-17 in vasculature.<br/>

Three tea catechins inhibit contraction of vascular smooth muscle

256th National Meeting (2018)

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