Recently, a number modified DNA (M-DNA) that can synthesize long M-DNAs have been identified. While these discoveries have enabled new applications of M-DNA, these enzymes typically have poor fidelity, possessing error rates several orders of magnitude worse than native enzymes. However, to-date, efforts to both quantify error rate, as well as experimental approaches towards understanding the origin of the poor fidelity of M-DNA polymerases, have been limited. Here, we use a high-throughput sequencing assay to characterize the error rate, error spectrum, and sequence bias of leading M-DNA polymerases. We also characterize the importance of individual mutations on fidelity, beginning to experimentally address the origins of fidelity.

Characterization of modified-DNA polymerase fidelity

256th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.