Metal ions interact over a range of affinities and specificity with biomembranes.  The interaction affects many properties and functions of lipid bilayers and associated biomolecules.  Measurement and characterization of ion binding to lipids is often difficult owing to the low affinity of the interaction and the analytical complications associated with dynamic and polydisperse liposome structures.  This presentation describes the use of colloid-supported lipid bilayers as stationary phases for affinity chromatography measurements of ion binding to lipids and bilayer-embedded ionophores.  The operational parameters and material development of the colloidal stationary phases are explained, and data highlighting the critical factor that the support structure plays in measuring lipid phase-sensitive binding interactions are shown.  Results for group II ions binding to different lipid classes and group I ions binding to membrane ionophores are presented, and a comparison is made to values determined using other techniques where available.  Chromatography provides a direct measure of affinity and enables measurements under physiologically relevant conditions.  This work describes our current understanding of the potential and limitations of the methodology for the study of ion-binding at lipid membranes.        <br/>

Affinity chromatography measurements of metal ion binding to lipid membranes

256th National Meeting (2018)

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