K562s are an erythrocyte-producing cell line, commonly used to study cholesterol and lipid transport. The goal of this work is to quantify lipid components in K562 cells by gas chromatography-mass spectroscopy. This analytical tool is needed for a subsequent dietary intervention study, measuring lipids in human subjects following their consumption of whole eggs, egg yolks or egg whites. Previous research has shown that eggs uniquely affect lipid composition and specifically cholesterol efflux capacity. Consequently, cholesterol was used to optimize parameters for gas chromatography. Cholestane, a closely related sterol, was used as an internal standard. Conveniently, the instrument’s mass spectrometer allowed for easy peak identification. The limit of detection and linear signal range were determined for the instrument. The knowledge gained from this work enables the analysis of cell culture samples and finally clinical samples obtained from the dietary intervention study.

Lipid profiling in K562 human leukemia cells via gas chromatography-mass spectrometry

256th National Meeting (2018)

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