We introduce biomolecular payloads into cells for gene editing at high throughput for off-the-shelf solutions targeting hemoglobinopathies, immune diseases, and cancers. We circumvent the need for viral transfection and electroporation, both of which have significant disadvantages in safety, throughput, cell viability, and cost. Mechanical deformation can make cell membranes transiently porous and enable gene-editing payloads to enter cells. These methods use specific chemical functionalization and control of surface contact and adhesion in microfluidic channels. Likewise, penetration of reproducibly nanomanufactured, loaded sharp features can introduce these packages into individual or many cells. We discuss our progress with these approaches and the methods that we use to quantify success.

Nanotechnology approaches to biological cellular therapies

256th National Meeting (2018)

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