Accurate DNA replication and transcription are critical for the survival of all organisms. DNA damage can interfere with replication efficiency and fidelity. DNA damage can also cause the transcription elongation complex to stall on the DNA and prevent DNA repair from occurring. The transcription-repair coupling factor Mfd can rescue stalled transcription and recruit DNA damage repair proteins to repair the DNA lesion. It is hypothesized that the Y-family polymerase DinB which is involved in bypassing DNA damage is one of these proteins recruited by Mfd. The protein-protein docking server ClusPro 2.0 was used to generate potential docking poses which were then analyzed to determine the most likely interaction. Several possible configurations have been identified, which will now be probed biochemically using protein truncations and mutations at the predicted sites of interaction.<br/>

Prediction of the interaction region between the Y-family polymerase DinB and the transcription-repair coupling factor Mfd in <i>E. coli</i>

256th National Meeting (2018)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Prediction of the interaction region between the Y-family polymerase DinB and the transcription-repair coupling factor Mfd in E. coli