The alkyl chains of fluorescent lipids insert into membranes and are used to understand their biophysical properties. In this study we used a modified dialkylaminostyryl fluorescent lipid, 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide (DiA), replacing the I<sup>-</sup> counterion with the Cl<sup>-</sup> anion to increase hydration of the polar head and to enable self-assembling in water and formation of vesicles. Vesicles composed of DMPC/DiA, DPPC/DiA, DSPC/DiA, DMPE/DiA, DPPE/DiA and DSPE/DiA have been prepared in mole ratios between 100/0 to 0/100, in order to investigate the effects of chain length and headgroup type on chain packing and phase separation in these mixed amphiphile systems, using nanocalorimetry and fluorescence data, as well as Langmuir-Blodgett trough measurements on monolayers.

Chain length and headgroup dependence of phase separation in mixed vesicles of DiA and phosphatidyl choline

256th National Meeting (2018)

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