GreenLight Biosciences has developed a scalable process for the production of low-cost RNA for agricultural applications. At the outset of development, a technoeconomic model was constructed and used to guide the R&D required to take GreenLight’s proprietary cell-free RNA production pathways from nascent concepts to a cost-effective, scalable process. Key cost drivers were identified from model sensitivity analyses, and R&D tasks were established and organized to assess and improve on those cost drivers in order to meet economic targets. The efforts were organized into three modules. The first module comprised strain engineering and fermentation development and sought to maximize the production of enzymes and template plasmid used in GreenLight’s proprietary RNA production reaction. The second module comprised recovery process development and sought to define recovery processes for the reaction additives, while minimizing losses. The third module comprised pathway engineering and biochemistry and sought to improve the yield and productivity of the RNA production reaction. This talk will describe how these R&D modules were defined using the model and how these modules were integrated to achieve a cost-effective, scalable RNA production process.

Low-cost and scalable production of RNA via cell-free bioprocessing

256th National Meeting (2018)

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