Exposure to ultraviolet B (UVB) can cause skin photoaging that is associated with upregulation of matrix metalloproteinases (MMPs) and downregulation of collagen synthesis. Perilla seed meal is a by-product of perilla oil production. Unsaponifiable matters (USM) from perilla seed meal contain bioactive phytochemicals such as tocopherols, tocotrienols, phytosterols, and policosanols and they are known to have antioxidant and health-promoting properties. In this study, we investigated the protective effects of USM against UVB-induced photoaging in human skin fibroblasts (Hs68 cells). We found that USM significantly reduced UVB-induced reactive oxygen species generation by 19, 24.1, and 27.1% at the concentration of 1.0, 2.5, and 5 ug/ml, respectively, compared to UVB irradiation (30 mJ/cm<sup>2</sup>) alone. USM attenuated UVB-induced MMP-1 and MMP-3 mRNA expression by suppressing phosphorylation of mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1). UVB exposure led to a 49.4% reduction in collagen synthesis. However, USM resulted in a dose-dependent restoration of the collagen synthesis through stimulation of growth factor β (TGF-β)/Smad2/3 pathways. These findings indicate that USM exhibits anti-photoaging activities and, thus, could be used for the potential application of cosmoceutical preparations.

Unsaponifiable matters from perilla seed meal protect against UVB-induced photoaging and promote collagen synthesis via stimulation of TGF-β/smad and inhibition of MAPK/AP-1 in human skin fibroblasts

2019 Spring National Meeting

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