Host cell proteins (HCPs) are endogenous impurities that can co-purify with several biological Drug Substances. High-throughput robotic screens can be utilized as an initial screening method to evaluate multiple chromatography resins, varying pH and salt concentrations in order to identify optimal resins and operating conditions for removal of problematic or over expressed host cell proteins in downstream purification processes. In these studies, purified host cell proteins were screened over several ion exchange, multimodal, and hydrophobic interaction chromatography (HIC) resins. Kp robotic screens were used to evaluate their binding interactions on the resins independent of a target antibody or protein. Using the Kp screen data, contour plots/impurity maps were generated that map the partition coefficients (Kp) of each of the host cell proteins screened. This poster demonstrates the Kp screens that were performed and illustrates the impurity maps that were generated to show the binding behavior of the different host cell proteins over various chromatography resins and conditions.

Mapping purified host cell proteins via high-throughput robotic screens

2019 Spring National Meeting

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